17 March 2020 - Measuring non productive interactions of enzymes with lignin

17 March 2020 - Measuring non productive interactions of enzymes with lignin

In lignocellulosic biomass, a sustainable source of energy and chemicals to replace fossil carbon, the activity of enzymes is limited by the appearance of non-specific interactions with lignin during the hydrolysis process, which maintains enzymes far from their substrate (cellulose and hemicelluloses). Characterization of these complex interactions is thus an important challenge.

Designing quantitative measurements is the goal of this article, resulting from the Lignoprog project funded by the ANR (2014-2018) and gathering FARE lab, PICT microscopy plateform and UGSF lab in Lille. The article describes a protocol and highlights in a video all the critical steps to follow! An original approach based on the measurement of lignin fluorescence lifetime is the core of this protocol which should allow the selection of enzymes less prone to these non-productive interactions to increase their efficiency.

Read: Terryn C, Habrant A, Paës G, Spriet C (2020) Measuring Interactions between Fluorescent Probes and Lignin in Plant Sections by sFLIM Based on Native Autofluorescence. JoVE, e59925. http://doi.org/10.3791/59925

Contact: Dr Gabriel Paës, gabriel.paes@inrae.fr

Modification date : 06 June 2023 | Publication date : 16 March 2020 | Redactor : G. Paës