23 January 2018 - Tracking enzymatic deconstruction of plant cell walls at synchrotron

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23 January 2018 - Tracking enzymatic deconstruction of plant cell walls at synchrotron

23 January 2018 - Tracking enzymatic deconstruction of plant cell walls at synchrotron

Combining microscopy with UV and infrared synchrotron beamlines allows in situ tracking of the boosting effect of a lytic polysaccharide monooxygenase (LPMO) on the deconstruction of miscanthus cell walls by cellulases.

This project comes from a close collaboration between FARE lab, DISCO and SMIS beamlines from SOLEIL synchrotron near Paris and BBF lab in Marseille. Using in situ imaging by synchrotron UV fluorescence, we have shown that the addition of a lytic polysaccharide monooxygenase (LPMO) from AA9 family to cellulases cocktail improves the progression of enzymes in delignified Miscanthus x giganteus as observed at tissular levels. In situ chemical monitoring of cell wall modifications performed by synchrotron infrared spectroscopy during enzymatic hydrolysis demonstrated that the boosting effect of the AA9 LPMO was dependent on the cellular type indicating contrasted recalcitrance levels in plant tissues. Our study provides a useful strategy for investigating enzyme dynamics and activity in plant cell wall to improve enzymatic cocktails aimed at expanding lignocelluloses biorefinery.

Read: Chabbert B, Habrant A, Herbaut M, Foulon L, Aguié-Béghin V, Garajova S, Grisel S, Bennati-Granier C, Gimbert-Herpoël I, Jamme F, Réfrégiers M, Sandt C, Berrin JG, Paës G. Action of lytic polysaccharide monooxygenase on plant tissue is governed by cellular type. Scientific Reports 2017, 7, 17792. DOI

Contacts: Dr Brigitte Chabbert, brigitte.chabbert@inra.fr
               Dr Gabriel Paës, gabriel.paes@inra.fr

Modification date : 06 June 2023 | Publication date : 22 January 2018 | Redactor : B. Chabbert / G. Paës