27 February 2023 - Method for automated morphological quantification of plant cells

27 February 2023 - Method for automated morphological quantification of plant cells

27 February 2023 - Method for automated morphological quantification of plant cells

Lignocellulosic biomass is a complex network of polysaccharides and lignin that requires a pretreatment step to overcome recalcitrance and optimize valorisation into biobased products. Pretreatment of biomass induces chemical and morphological changes. Quantification of these changes is critical to understand biomass recalcitrance and to predict lignocellulose reactivity.

In this study, we propose an automated method for the quantification of chemical and morphological parameters through fluorescence macroscopy, which was applied on wood samples (spruce, beechwood) pretreated with steam explosion.

Results for fluorescence macroscopy highlighted the impact of steam explosion on these samples: fluorescence intensity was highly altered, especially for the most severe conditions. We also showed that lumens area and circularity could be considered as complementary markers of cell deformation, and that fluorescence intensity of the cell walls could be related to morphological changes and to the conditions of pretreatment.

The developed procedure thus allows simultaneous and effective quantification of morphological parameters and fluorescence intensity of the cell walls. This approach can be applied to fluorescence macroscopy as well as other imaging techniques and provides encouraging results towards the understanding of biomass architecture.

This work was made possible through a collaboration between FARE lab and PLBS in Lille with a funding from the European Union and Région Grand Est through FEDER (Hy-C-Green project).

Read: Audibert E, Lebas B, Spriet C, Habrant A, Chabbert B and Paës G (2023) Automated quantification of fluorescence and morphological changes in pretreated wood cells by fluorescence macroscopy. Plant Methods 19, 16. http://doi.org/10.1186/s13007-023-00991-6

Contact : Dr Brigitte Chabbert, brigitte.chabbert@inrae.fr

               Dr Gabriel Paës, gabriel.paes@inrae.fr

Modification date : 06 June 2023 | Publication date : 23 February 2023 | Redactor : G. Paës