2024-07-17 Article LPMO.jpg
A novel imaging approach to monitor the action of oxidative enzymes at the cellular scale

A novel imaging approach to monitor the action of oxidative enzymes at the cellular scale

Oxidative enzymes play an important role in degrading lignocellulosic biomass, but the modifications they carry out are often difficult to localize and quantify in situ on a cellular scale.

As part of Amandine Leroy's PhD thesis between the BIA and FARE laboratories, with the collaboration of the BBF laboratory and the BIBS platform, we developed and applied a MALDI-MS imaging method to detect oxidized oligosaccharides released by an oxidative cellulose enzyme (LPMO) on corn stalk tissues, at the cellular scale.

The results obtained enabled us to image the action of LPMO and measure the spatial variation and relative abundance of oxidized and non-oxidized oligosaccharides. LPMO thus has a targeted action linked to the composition and organization of plant cell walls.

This approach opens up possibilities for better studying and understanding the synergistic effects of various hydrolytic and oxidative enzymes on lignocellulosic wall degradation, with a view to improving enzyme cocktails for biotechnological applications.

Amandine Leroy's thesis was co-financed by the Région Grand Est and the Communauté d'agglomération du Grand Reims.

Read: Leroy A, Fanuel M, Alvarado C, Rogniaux H, Grisel S, Haon M, Berrin J-G, Paës G*, Guillon F. In situ imaging of LPMO action on plant tissues. Carbohydrate Polymers 2024, 343, 122465. https://doi.org/10.1016/j.carbpol.2024.122465

Contact: Dr Gabriel Paës, gabriel.paes@inrae.fr

Modification date: 17 July 2024 | Publication date: 17 July 2024 | By: G. Paës