Design of a Peroxidase-Based Gas Diffusion Electrode

Design of a Peroxidase-Based Gas Diffusion Electrode

Design of a Peroxidase-Based Gas Diffusion Electrode for Bioelectroenzymatic Applications

We report here the design and application of an all-in-one gas diffusion electrode (GDE) combined with surface immobilized horseradish peroxidase (HRP) capable of in situ generation of hydrogen peroxide (H2O2) from air and simultaneous oxidation of a model substrate within a single-cell electrochemical reactor. Carboxyl-functionalized multiwalled carbon nanotubes were employed as an electrocatalyst for oxygen reduction reaction. We investigated and compared two immobilization strategies to obtain HRP-modified biocathodes. Our findings illustrate prospects for integrating GDE and surface-bound peroxidases for H2O2-dependent electroenzymatic reactions, offering a promising platform for diverse applications in bioelectrosynthesis.

Mishyn V, Carducci NGG, Hickey DP, Abdellaoui S. 2025. Electroanalysis 37: e12015; doi.org/10.1002/elan.12015